Ethnomedicinal Knowledge Verification for the Antidiarrheal and Antioxidant Effects of Rhus chinensis Mill. Fruits with Identification of Thirty Constituents

Background: Ethnobotanical survey in the rural villages Nepal revealed that fruits of Rhus chinensis Mill. have been using for treatment diarrhea and dysentery. Objective: To evaluate antimicrobial antioxidant effects, identification chemical constituents R. chinensis. Materials Methods: Phytochemical screening was performed on hexane 70% methanolic extracts sample followed by gas chromatographymass spectrometry (GC-MS). Total phenolic content (TPC) estimated Folin-Ciocalteu method. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical hydrogen peroxide scavenging assays were used to capacity. Antibacterial effect estabilished Agar well diffusion assay. Results: A total 30 compounds belonging phenolics, anhydrides, aldehydes, fatty acids hydrocarbons identified extracts. The TPC value 123.52±1.29 mg GAE/g dry extract estimated. IC50 135.54±0.82 μg/mL calculated DPPH Scavenging 42.69±0.1% 63.20±1.48% at 100 concentration maximum zone inhibition (ZOI) observed 23.00±0.57 mm against Escherichia coli loading dose 5 extract. Conclusion: All together fruits. efficiently inhibited growth E. Shigella dysenteriae verifying knowledge. At same time, displayed efficient activity. phytochemicals responsible these activities.